Transformation Process Research Articles

TurboID mapping reveals the exportome of secreted intrinsically disordered proteins in the transforming parasite Theileria annulata.

Theileria annulata is a tick-transmitted apicomplexan parasite that gained the unique ability among parasitic eukaryotes to transform its host cell, inducing a fatal cancer-like disease in cattle. Understanding the mechanistic interplay between the host cell and malignant Theileria species that drives this transformation requires the identification of responsible parasite effector proteins. In this study, we used TurboID-based proximity labeling, which unbiasedly identified secreted parasite proteins within host cell compartments. By fusing TurboID to nuclear export or localization signals, we biotinylated proteins in the vicinity of the ligase enzyme in the nucleus or cytoplasm of infected macrophages, followed by mass spectrometry analysis. Our approach revealed with high confidence nine nuclear and four cytosolic candidate parasite proteins within the host cell compartments, eight of which had no orthologs in non-transforming T. orientalis. Strikingly, all eight of these proteins are predicted to be highly intrinsically disordered proteins. We discovered a novel tandem arrayed protein family, nuclear intrinsically disordered proteins (NIDP) 1-4, featuring diverse functions predicted by conserved protein domains. Particularly, NIDP2 exhibited a biphasic host cell-cycle-dependent localization, interacting with the EB1/CD2AP/CLASP1 parasite membrane complex at the schizont surface and the tumor suppressor stromal antigen 2 (STAG2), a cohesion complex subunit, in the host nucleus. In addition to STAG2, numerous NIDP2-associated host nuclear proteins implicated in various cancers were identified, shedding light on the potential role of the T. annulata exported protein family NIDP in host cell transformation and cancer-related pathways.IMPORTANCETurboID proximity labeling was used to identify secreted proteins of Theileria annulata, an apicomplexan parasite responsible for a fatal, proliferative disorder in cattle that represents a significant socio-economic burden in North Africa, central Asia, and India. Our investigation has provided important insights into the unique host-parasite interaction, revealing secreted parasite proteins characterized by intrinsically disordered protein structures. Remarkably, these proteins are conspicuously absent in non-transforming Theileria species, strongly suggesting their central role in the transformative processes within host cells. Our study identified a novel tandem arrayed protein family, with nuclear intrinsically disordered protein 2 emerging as a central player interacting with established tumor genes. Significantly, this work represents the first unbiased screening for exported proteins in Theileria and contributes essential insights into the molecular intricacies behind the malignant transformation of immune cells.

Hindrance or facilitator? The economic consequences of national demonstration eco-industrial parks in China.

Many countries attach great importance to the green, low-carbon, and circular development of industrial parks. China is one of them and has entered an exploration journey of national demonstration eco-industrial parks (NDEIPs). However, the impact of the transformation of industrial parks into NDEIPs on local economic development still remains a mystery. To address this issue, we develop an empirical study using a combination of the multi-period difference-in-differences method and the propensity score matching method based on the panel data for 266 cities in China from 2001 to 2021. The results show that industrial parks becoming NDEIPs promotes cities' economic development. This conclusion still holds after a series of robustness tests, such as the reverse causality test and the placebo test. Moreover, the park heterogeneity tests show that the economic consequences vary according to differences in levels, industry types, life cycle phases, and the degree of foreign firm agglomeration. The city heterogeneity tests show that the economic consequences differ based on administrative levels, innovation capabilities, technology industrialization, and environmental friendliness. The spatial heterogeneity tests show that the economic consequences differ according to geographical location and whether situated in the Yangtze River Economic Belt. The policy upgrading heterogeneity tests show that the economic consequences differ during the process of policy upgrading and transformation. In addition, the mechanism tests reveal that green innovation, human capital level, and firm attractiveness mediate the relationship between industrial parks becoming NDEIPs and cities' economic development. This study provides a new perspective for understanding the economic effects of the transformation of industrial parks into NDEIPs, and provides a reference for the government on how to maximize these economic effects.

Sequential oxidation-composting-phytoremediation treatment for the management of an oily sludge from petroleum refinery

Oily sludges are generated in large quantities in petroleum refinery wastewater treatment plants. Given their complex composition, they are classified as hazardous waste. Selecting a single treatment technique for their remediation is challenging.This work aims to assess the extent of composting followed by phytoremediation on an oily sludge from an API separator unit, pre-treated by chemical oxidation with alkaline activated persulfate (PS). 18% of total petroleum hydrocarbons (TPH) were determined by IR spectroscopy. The aliphatic hydrocarbon content was 4714 ± 250 ppm by GC-FID, and aromatics were not detectable, suggesting a high amount of non-chromatographable complex hydrocarbons. The density of generalist and hydrocarbon-degrading populations of the oily sludge estimated by quantitative polymerase chain reaction (qPCR) evidenced an autochthonous microbiota with hydrocarbon-degrading capacity. The oxidative treatment with PS removed 31% of the TPH determined by IR after 20 days. The significant reduction of the native bacterial community was counterbalanced by coupling a composting treatment. Co-composting the sludge with goat manure and oat straw produced, after a year, a 96% reduction in TPH content, regardless of the oxidative pretreatment. Organic matter transformation was evidenced by the decrease of dissolved organic carbon (DOC) and the variation in E4/E6 ratio. The matrices obtained of composting were used as substrates for phytoremediation for 4 months. Ryegrass seeds were planted in both PS-treated and untreated sludge substrates. The presence of the plant grown in the pre-oxidised and composted substrate resulted in a higher aerial biomass of ryegrass (67%), an increase in enzymatic activities, and higher concentration of DOC, although without evidence of additional dissipation of TPH.The dynamics of the bacterial communities of the different substrates generated during the biological treatment were analyzed by Illumina NovaSeq DNA sequencing of 16S rRNA amplicons. The findings mirrored a succession compatible with that described in contaminated matrices, but also in other non-contaminated ones.According to these findings, an organic matter transformation process occurred, which included the complex hydrocarbons of the oily sludge, resulting in an active substrate that promoted the retention of nutrients and water and provided the necessary support for plant development.

Four-bar linkage reconfigurable robotic wheel: Design, kinematic analysis, and experimental validation for adaptive size modification

Abstract This article presents the development of a robot capable of modifying its size through a wheel reconfiguration strategy. The reconfigurable wheel design is based on a four-bar retractable mechanism that achieves variation of the effective radius of the wheel. A reconfiguration index is introduced based on the number of retractable mechanisms that predicts the radius of configuration according to the number of mechanisms implemented in the wheel. The kinematics of the retractable mechanism is studied to determine the theoretical reconfiguration radius during the transformation process, it is also evaluated numerically with the help of the GeoGebra software, and it is validated experimentally by image analysis using the Tracker software. The transformation process of the robot is investigated through an analysis of forces that consider the wheel in contact with the obstacle, the calculation of the wheel torque and the height of the obstacle to be overcome are presented. On the other hand, the experimental validation of the robot reconfiguration process is presented through the percentage of success shown by the robot to overcome obstacles of 50, 75, 100 and 125 mm. In addition, measurements of energy consumption during the transformation process are reported. Reconfigurable wheels, capable of adapting their size, offer innovative solutions to various challenges across different applications such as robotic exploration and search and rescue missions to industrial settings. Some key issues that these wheels can address include terrain adaptability enhancing a robot’s mobility over uneven surfaces, or obstacles; enhanced robotic design; cost-effective design; space efficiency; and versatility in applications.

Echoes of silence.

Communication is an integral part of nursing practice-with patients and their relatives, other nurses and members of the healthcare team, and ancillary staff. Through interaction with the 'other', language and silence creates and recreates social realities. Acceptance, rejection or modification of social realities depends on what is expressed and by whom. Narratives that are offered can tell of some experiences and not others. Some nurses choose to be silent while others are silenced. In nursing situations recognising and allowing silence to speak is a challenging but uniquely personal experience that embraces reflection in and on experiences, practice and self as a person and a professional. If enabled and truly heard, silence can speak more loudly than the hubbub of daily practice, allowing us to collectively question and challenge inherent assumptions and biases as professionals, and as a profession. Through a microcosm of Newly Graduated Nurses' lived experiences of nursing situations and expressions of silence individuals' discomfort and private efforts to ascribe meaning to experiences are reflected on. Returning to silence is to return to a constant process of professional transformation that can enable ways of knowing and being that can reform our profession from within and enable us to cast off shackles that bind us to a shameful cultural underbelly.

Impact of coating and microstructure on wear resistance of tool steels for wood cutting: a novel approach to quantification and analysis of wear-related damages

Squaring, a wood transformation process, is an operation which consists of introducing the logs into a squaring machine which then uses sharp tools to cut the wood into pieces with high surface quality. Tool steels used in this process experience significant wear, damaging the wood surface and hence leading to substantial scrape rate. This study investigates the wear resistance of three tool steels specifically designed for wood cutting applications: modified AISI A8, modified steels with 0% and 1% tungsten, and powder metallurgy prepared W360 steel. Furthermore, the influence of a PVD coating on the wear resistance of the three alloys was investigated. ASTM G65 abrasive wear tests were conducted using the dry sand/rubber wheel abrasion test. A methodology using a non-contact 3D measurement system and specialized software was developed, allowing for a thorough quantitative assessment of the wear of these steels. The results revealed that the coated A8mod + 1%W steel exhibits the best resistance among the coated steels. Despite the excellent intrinsic resistance of W360 steel, the coating did not provide a significant improvement for this steel, showing only a 10% reduction in wear. Microstructural analysis revealed that the predominant wear mechanisms were abrasion and impact. The relative performance of each steel was quantified and is reported. Field trials conducted under actual cutting conditions, indicate the superiority of W360 steel in terms of resilience to wear and impacts compared to other tested alloys, while confirming the effectiveness of surface treatments in mitigating material wear. However, A8 steel modified with 1% tungsten exhibits increased wear under coating.

Transformation and Degradation of PAH Mixture in Contaminated Sites: Clarifying Their Interactions with Native Soil Organisms

Soil contamination of polycyclic aromatic hydrocarbons (PAHs), especially caused by the mixture of two or more PAHs, raised great environmental concerns. However, research on the migration and transformation processes of PAHs in soils and their interactions with native communities is limited. In this work, soil samples from uncontaminated sites around the industrial parks in Handan, Hengshui, and Shanghai were artificially supplemented with three concentrations of anthracene (Ant), 9-chloroanthracene (9-ClAnt), benzopyrene (BaP), and chrysene (Chr). Ryegrass was planted to investigate the degradation of PAHs and its interaction with native soil organisms in the constructed ryegrass–microbe–soil microcosmic system. The bacterial and fungal communities in soil were affected by PAHs; their species diversity and relative abundance changed after exposure to different concentrations of PAHs, among which Lysobacter, Bacillus, Pseudomonas, and Massilia bacteria were correlated to the degradation of PAHs. On the 56th day, the contents of BaP, Chr, and Ant decreased with the degradation process, while the degradation of 9-ClAnt was limited. Nineteen intermediates, including hydroxylation and carboxylated compounds, were identified. The present research would help clarify the potential interactions between PAHs and native organisms in contaminated sites, providing fundamental information for evaluating the transformation risks of PAHs in the natural environment.

From genetic mosaicism to tumorigenesis through indirect genetic effects.

Genetic mosaicism has long been linked to aging, and several hypotheses have been proposed to explain the potential connections between mosaicism and susceptibility to cancer. It has been proposed that mosaicism may disrupt tissue homeostasis by affecting intercellular communications and releasing microenvironmental constraints within tissues. The underlying mechanisms driving these tissue-level influences remain unidentified, however. Here, we present an evolutionary perspective on the interplay between mosaicism and cancer, suggesting that the tissue-level impacts of genetic mosaicism can be attributed to Indirect Genetic Effects (IGEs). IGEs can increase the level of cellular stochasticity and phenotypic instability among adjacent cells, thereby elevating the risk of cancer development within the tissue. Moreover, as cells experience phenotypic changes in response to challenging microenvironmental conditions, these changes can initiate a cascade of nongenetic alterations, referred to as Indirect non-Genetic Effects (InGEs), which in turn catalyze IGEs among surrounding cells. We argue that incorporating both InGEs and IGEs into our understanding of the process of oncogenic transformation could trigger a major paradigm shift in cancer research with far-reaching implications for practical applications.

Rethinking Energy Geopolitics: Towards a Geopolitical Economy of Global Energy Transformation

ABSTRACT We are in the midst of a global energy system transformation (GEST) which is rewiring the world economy, opening new axes of political contestation, and revolutionising the energetic basis of human civilisation. Energy geopolitics has not yet reconciled itself to this challenge. The field has traditionally been preoccupied with the dependence of Western states on cross-border flows of fossil fuels. More recently, efforts have been made to prospectively map out what the geopolitics of a fully renewable world might look like. What both literatures miss, however, is the very fact of the GEST: that we are living through a changing and contested process of global transformation, across interacting high- and low-emissions systems, whose contours are open and actively constructed over time. In this paper, we start to develop a provisional framework to make sense of the GEST, that is able to capture the full scale of the transformation, and its dynamic, contingent, constructed nature. We attend to three areas of geopolitical economy: the wide-ranging material dimensions of the transformation, its geographical space-making, and its conflict-ridden political economy. We then apply this framework to two case studies, one looking at the fraught role of fossil gas as a ‘transition fuel’, the other at lithium-ion batteries.

Navigating Ritual Resilience: The Interplay of Change and Continuity in the Shaolin Monastery During the COVID-19 Pandemic

The global upheaval caused by the COVID-19 pandemic introduced considerable perturbations to the religious life and ritual observances within most religious communities around the world. This article delves into the resilience and adaptability of ritual practices within the Shaolin Monastery of China during the five-month COVID-19 quarantine spanning from 23 January to 22 June, 2020. Employing ethnographic methods and grounded in anthropological theory, the study examines the interplay between ritual as a static social fact and as a dynamic, adaptable process, within the context of the Shaolin monastic life. It underlines the intricate interplay between static and dynamic elements in ritual practice, casting light on the Shaolin community’s response to the pandemic. The study discerns dichotomies responsible for the critical forces underlying complex dynamics intrinsic to the Shaolin community’s religious life, namely: religious–secular, isolation–social engagement, discipline–freedom, tradition–innovation, and individual–collective. Tracking the evolutions and consistencies within these dual relationships enabled casting light on the transformative processes that were set in motion and identifying the elements of the religious life that have undergone change and those that have remained constant. This exploration offers a unique glimpse into the mechanisms through which resilience was enacted with the strategies that enabled the community to maintain, adjust and innovate its ritual practices amidst the tribulations presented by the crisis.

Challenges and Solutions in the Digital Transformation of "Specialized, Refined, Unique, and New" Small and Medium Enterprises

Against the backdrop of the Digital China initiative, exploring how "Specialized, Refined, Unique, and New" (SRUN) enterprises can effectively undergo digital transformation to enhance innovation capabilities is a crucial and urgent topic. For these enterprises, accelerating digital transformation is key to addressing contemporary challenges, improving efficiency, reducing costs, showcasing unique advantages, and leading the trend of intelligent manufacturing. Throughout the transformation process, these enterprises face multiple dilemmas and challenges: despite a strong willingness to transform, they lack corresponding capabilities; infrastructure is improving, but the technological environment is not yet ideal; willingness to collaborate is increasing, but effective synergy is lacking; policy support is growing, but needs to be more precise. In order to better promote the digital transformation of SRUN enterprises, appropriate strategies must be adopted to address these challenges. This includes enhancing awareness and strengthening talent development, improving infrastructure, optimizing the transformation environment, fostering a favorable digital ecosystem, enhancing collaborative cooperation, and optimizing institutional design to elevate service standards. These measures collectively provide a pathway for SRUN enterprises to drive digital transformation.

Architectural and Urban Changes in a Residential Environment—Implications for Design Science

Current spatial changes involving broad urban landscapes affect people’s perceptions of their surroundings, sense of place, and attachment to a place, constituting a disruption to these. As a result, on a social scale, they translate into people’s well-being. The following study of the impact of large-scale architectural and urban developments on the place of residence is based on the assumption that physical space determines the quality of life in the living place and the changes in the process condition residents to adapt to their new surroundings—based on the three pillars of place, people, and process (3Ps). The article consists of two parts. The first is theoretical, which conceptualises spatial change based on the theory of human dependence on place. The second part–an empirical study—discusses the transformation of the post-industrial area of Bicocca (Milan), which, 40 years after the intervention, has led to conclusions and recommendations for urban planning. The results demonstrate the different sensitivities of the urban areas to the process of functional–spatial change, the essence of the accessibility of public space, public facilities, and transport infrastructure. The planning process can positively influence social adaptation to spatial change mitigation. Residential areas may be subject to additional protection procedures. The study is relevant to a sustainable planning process in the inevitable transformation of urban areas. The interdisciplinary nature of the issue prompts the integration of research findings and knowledge transfer in the socio-technological subsystem (STS).

Traditional agencies on bridges: How is digital transformation changing business models?

AbstractDigital technologies produce new strategic and organizational challenges for incumbent firms that require major digital transformation (DT) processes, leading to the evolution of business models (BMs). Contributing to the research stream on DT and BM evolution, this study explores which technologies have the most relevant effect on core BM dimensions, i.e., value proposition, value creation, and value capture, by investigating both the consequent themes and intensities of change in these dimensions. A valuable context for analysis was chosen: the advertising industry and traditional communication agencies, namely, creative and media agencies. Based on 38 in-depth interviews with senior communication professionals and qualitative content analysis, we propose a comprehensive reading of (1) the impact of specific technologies on different BM dimensions and components and (2) the themes and intensities of change in creative and media agencies’ BM dimensions. Finally, we outline an interpretative framework, identifying two different paths for incumbents: outside-in BM adaptation and inside-out BM innovation. This study therefore offers two academic contributions. First, it overcomes the vagueness of previous investigations that considered the effects of digital technologies in general terms, and it also resolves the narrow scope of research that focused only on the impact of one specific technology. Second, by deepening the understanding of the joint evolutions and intensities of change across all three BM dimensions, this study has widened the limited scope of previous contributions on one BM dimension. By doing so, it offers a comprehensive reading of BM dynamics due to DT.

Lexis and Grammar Specifics of the English Language in Africa

This research work deals with the lexical and grammatical features of the English language variant widely used in Africa. The importance of the use of British English is also taken into account, as well as its transformational processes due to the interaction and mutual influence of local dialects on its structure. Examples of neologisms and modified lexical units are given, based on the influence of local culture and the lack of an equivalent in the recipient language. The article pays attention to the grammatical aspects of language in the functioning of phrasal, modal and prepositional verbs, and also reveals the peculiarities of articles and pronouns use due to the influence of autochthonous languages. The aim of the research work is to identify the specifics of the linguistic and cultural adaptation of the English language in Africa and systematize the lexical and grammatical aspects of the English language as the territorial variant type. The choice of working methods is determined by the goals and objectives of the study. In the research work the method of metalinguistic analysis, methods of contextual and definitional analysis, methods of systematization and classification of linguistic material are used. As a result of the work the high productivity of the realities functioning in autochthonous languages in the lexical structure of the English variant in African countries and the lexical borrowings of terms from autochthonous languages are revealed. This trend is also emphasized in the borrowing of entire lexical phrases from the thesaurus of native languages. With regard to the grammatical part, a significant deviation from British English is emphasized. The research work results in such grammatical changes as: the use of auxiliary verbs together with semantic ones; the functioning of a simplified form of modern forms of the verb; the productive use of uncountable nouns in speech in the form of a countable nouns; significant deviations from British English in the process of using articles, personal and possessive pronouns.

Water Tower Home transformation: bottom-up urban regeneration through a reality TV show

The article examines Wutopia Lab’s transformation of the Water Tower Home (shuita zhijia, also know as ‘House on the House’), a top-floor unit of a residential building converted from a water tower located in a Bulinli lilong neighbourhood in Shanghai. In 2015, the top-floor unit was transformed into a three-bedroom apartment, which was featured in a popular reality TV show Dream Home. Using the concept of ‘raumplan’ as the primary design strategy, the design deploys various heights and platforms to re-organise the internal space while largely maintaining the exterior appearance of the building. It deliberately avoided homogeneous visual control and accommodated the residents’ complex functional requirements within a highly restricted space. The research scrutinises various actors’ involvement during the production of the reality TV show and the transformation process. It highlights how the design team navigated the stakeholders’ complex needs and the rigid yet ambiguous policy related to the regeneration of Shanghai’s unofficial urban heritage. By reflecting upon the various formal and informal design practices in this structure and its eventual demolition, the article illustrates the dilemmas in bottom-up regeneration of the historic urban environment in contemporary China.

Transformative process theory

Abstract Transformative constitutionalism and process theory are generally seen as worlds apart. But they may be more compatible than we think. A transformative understanding of process is very broad, but it represents a natural extension of the line already being taken by contemporary process accounts intent on expanding the theory to fit global practice. It can help us to understand why an expansion based on and including a wider set of justiciable process concerns has proved difficult to limit. Conversely, transformative constitutionalism badly needs a better account of judicial restraint to balance its preoccupation with judicial boldness. Since it shares with process theory a deep concern with democracy, it can naturally draw on process accounts to understand its own limits. Democracy-seeking review, in aiming to build as well as protect democratic capacity, needs to be as concerned with restraint as intervention, depending on the context. Working out a transformative process theory is therefore at least an exercise instructive to either side, and it can offer a way to overcome divides that hamper global engagement with these core constitutional issues.

Head Teacher's Self-efficacy with Transforming Leadership for Changing School

In the context of Nepal, most of government-aided schools are poorly managed. They could not provide good achievement academically and non-academically. Head teacher's leadership is considered highly significant for the transformation of such schools. The schools are not getting expected change. However, there are very few schools that have been transformed considerably. This study explored the role of head teachers’ self-efficacy and their transformative leadership qualities in connection to the transformation of schools. Along with this, teachers' perception about the head teachers' leadership role and impact in the transformation process were also underpinned. This multiple case study of two government schools from Dhading district adopted qualitative method. The findings were illustrated in narrative and descriptive way. Research findings indicated the current situation of transformed schools, leadership effect, the head teachers' self-efficacy and transformative leadership qualities. The findings are helpful to head teachers, administrators, teachers, policy makers and school managers per se in order to transform schools.

How new rural elites facilitate community-based homestead system reform in rural China: A perspective of village transformation

In line with China's rural transformation, the homestead system reform (HSR) pilots have been launched to rectify adverse land-related issues by formalising and capitalising land property rights. In contrast with a government-led model prevalent among most national HSR pilots, a community-based approach has been experimented with and proven efficient in Yujiang County, southern China. It highlights a pivotal role of the endogenous and public-spirited activists who are locally called ‘new rural elites’ (NREs). From the perspective of village transformation processes, this paper establishes a theoretical framework and employs a case study in Yujiang to interpret the underlying rationale for NREs' involvement in HSR. The findings indicate that HSR, aiming at multi-level space reshaping and ‘three rights separation’ reform, is in alignment with the triple processes of village transformation (i.e., systemic, communal, and living). The NREs represent a cultural force for facilitating village transformation, which is underpinned by the capacity, channel and carrier endowed by urban-rural linkages, interpersonal value transmission and community social solidarity, respectively. The benevolent dominance of the NREs is jointly shaped by governmental empowerment and accountability, egalitarian property rights design and strong social solidarity. To expand NREs' benevolent roles, this study recommends aligning community empowerment with the improvements of community-based action capacity.

The Use of Spectroscopic Methods to Study Organic Matter in Virgin and Arable Soils: A Scoping Review

The use of modern spectroscopic methods of analysis, which provide extensive information on the chemical nature of substances, significantly expands our understanding of the molecular composition and properties of soil organic matter (SOM) and its transformation and stabilization processes in various ecosystems and geochemical conditions. The aim of this review is to identify and analyze studies related to the application of nuclear magnetic resonance (NMR) and electron paramagnetic resonance (EPR) spectroscopy techniques to study the molecular composition and transformation of organic matter in virgin and arable soils. This article is mainly based on three research questions: (1) Which NMR spectroscopy techniques are used to study SOM, and what are their disadvantages and advantages? (2) How is the NMR spectroscopy technique used to study the molecular structure of different pools of SOM? (3) How is ESR spectroscopy used in SOM chemistry, and what are its advantages and limitations? Relevant studies published between 1996 and 2024 were searched in four databases: eLIBRARY, MDPI, ScienceDirect and Springer. We excluded non-English-language articles, review articles, non-peer-reviewed articles and other non-article publications, as well as publications that were not available according to the search protocols. Exclusion criteria for articles were studies that used NMR and EPR techniques to study non-SOM and where these techniques were not the primary methods. Our scoping review found that both solid-state and solution-state NMR spectroscopy are commonly used to study the structure of soil organic matter (SOM). Solution-phase NMR is particularly useful for studying soluble SOM components of a low molecular weight, whereas solid-phase NMR offers advantages such as higher 13C atom concentration for stronger signals and faster analysis time. However, solution-phase NMR has limitations including sample insolubility, potential signal aggregation and reduced sensitivity and resolution. Solid-state NMR is better at detecting non-protonated carbon atoms and identifying heterogeneous regions within structures. EPR spectroscopy, on the other hand, offers significant advantages in experimental biochemistry due to its high sensitivity and ability to provide detailed information about substances containing free radicals (FRs), aiding in the assessment of their reactivity and transformations. Understanding the FR structure in biopolymers can help to study the formation and transformation of SOM. The integration of two- and three-dimensional NMR spectroscopy with other analytical methods, such as chromatography, mass spectrometry, etc., provides a more comprehensive approach to deciphering the complex composition of SOM than one-dimensional techniques alone.

Identifying the Crucial Biomarker of MASH-Related HCC.

This study aimed to explore the key oncogenic factor of metabolicassociated steatohepatitis (MASH) to hepatocellular carcinoma (HCC). We utilized four differential GEO datasets (GSE164760, GSE139602, GSE197112, and GSE49541) to identify the key oncogenic factor for MASH-related HCC. The differential genes were analyzed using the GEO2R algorithm online. The GEPIA online website was used to explore the expression of selected four genes (SPP1, GNMT, CLDN11, and THBS2). The genetic alterations in genes were estimated by the cBioPortal website. The Kaplan-Meier Plotter online database was applied to explore the prognostic value of SPP1. Univariate and multivariate Cox analyses were carried out to further confirm the prognostic value of SPP1. The GO and KEGG enrichment analysis exported associated pathways with SPP1 expression. The positively or negatively related immune cells and immune checkpoint expressions were identified through Pearson correlation analysis. The lipogenesis-associated proteins were detected using western blotting and fluorescence. The high-fat diet (HFD) mouse model was constructed, and liver samples were collected. SPP1, GNMT, CLDN11, and THBS2 were determined in the transformation process of MASH to liver fibrosis. SPP1 and GNMT were upregulated in the HCC tumor tissue. SPP1, in particular, had the potential to be the prognostic factor through Cox analysis. Remarkably, SPP1 was highly expressed in HCC compared to normal tissues in three independent datasets (GSE121248, GSE14520, and GSE45267). SPP1 is mainly involved in the amplification and deep deletion mutations. SPP1 was found to be strongly correlated with ANXA2 expression, and ANXA2 was also highly expressed in HCC with significant prognostic performance. Moreover, SPP1 was found to participate in the carcinogenic mechanism and correlate with immune cells and immune checkpoint expression. SPP1 knockdown suppressed the SREBP1 and FASN expressions and increased the SIRT1 expression in vitro. Moreover, the HFD model validated the upregulation of SPP1 in the fatty liver in vivo. SPP1 may be the key oncogenic factor for the transformation of MASH to HCC, and it could be a potential immunotherapeutic target in HCC.